m13 primer sequence

Use this program to produce a useful reference figure, particularly when you have designed a large number of primers for a particular template. GENEWIZ offers a variety of free universal primers for sequencing. The pUC/M13 Primers are used to sequence inserts cloned into the M13 vectors and pUC plasmids developed by Messing. M13 Fwd(GW) TGTAAAACGACGGCCAGT Use the malE primer (NEB# S1237S) on the 5´ side of the insert. M13 Primer M3 and M13 Primer M4 are forward DNA sequencing primers while M13 Primer RV is a reverse DNA sequencing primer. HPLC‐purified oligonucleotides were synthesized by Eurofins MWG Operon (Ebersberg, Germany). The primers are purified by gel electrophoresis or HPLC and supplied in sterile water. Simply select the primer and add it to the cart. Invitrogen™ M13 Forward (-20) Oligonucleotides complementary to a DNA template are necessary to prime DNA synthesis for sequencing reactions. ggt gac act ata gaa tac tca agc. The primer is a 23-base oligodeoxyribonucleotide of the sequence 5'-AGCGGATAACAATTTCACACAGG-3', with free hydroxyl groups at the 5'- and 3'-termini. Routine purity >90%; Sequence-verified via ESI-mass spectrometry* Obtain 10 µg of … Manufacturer: Invitrogen™ N52002 Catalog No. Free Universal Primers. (-29) M13 Reverse Primer Set is a single-stranded oligonucleotide with 5′-hydroxyl and 3′-hydroxyl ends and a selection of four fluorescent lables for use in polymerase chain reaction protocols (PCR). Sequence Manipulation Suite: PCR Primer Stats: PCR Primer Stats accepts a list of PCR primer sequences and returns a report describing the properties of each primer, including melting temperature, percent GC content, and PCR suitability. M13/pUC. All specific primers must be supplied by the client, but we have available free of charge a few common sequencing primers: M13(-21)F, M13(-46)R, T3, T7, and SP6. Primer Sequences. Hi, in my sequencing kit you are using only one sequencing primer either T7 or sp6. 5'AD: 5'- AGG GAT GTT TAA TAC CAC TAC -3' 3'AD: 5'- AGA TGG TGC ACG ATG CAC AG -3' 5AOX1: 5'- GAC TGG TTC CAA TTG ACA AGC -3' 3AOX1: 5'- GCA AAT GGC ATT CTG ACA TCC -3' A-FACTOR: Dumbrell, A. J., Ashton, P. D., Aziz… Order these from your oligo provider and your PCR is (almost) ready to sequence. Sequence: Length: Tm [°C] GC [%] 5'AACAGCTATGACCATGATTACG3' 22. To make use of these easy and free primers you’ll need to include them in the design of your locus specific PCR primers, simply add the M13 sequences to the 5′ ends. The minor coat protein P3 attaches to the receptor at the tip of the F pilus of the host Escherichia coli. If your template contains a poly A tail and you would like to sequence through it, please let us know before hand as we can use a "poly T mix" primer pool which has given good results with poly A regions. If you want to sequence the 3´ junction, the pUC/M13 primers that bind to the lacZ-a region (e.g. Primer Name Sequence Size (mer) Tm °C Remarks; 1. N52002. ReadyMade Primers include random hexamers, T7 promoter/terminator, M13 primers, 16S rRNA primers, and varieties of oligo dT that are available for same-day shipping. pUC/M13Primersは、Messingにより開発されたpUCベクターやM13ベクターにクローニングされたDNAのシークエンシングに使用します。プロメガのpGEM(R)-Z VectorやpGEM(R)-Zf Vectorのような、lacZ遺伝子を含むプラスミドにも使用できます。 No. Random Primers can ... 26-3000-01 M13/pUC (-20) 17mer 25 ug 26-3000-02 M13… In order to enable fast and convenient ordering of sequencing primers that are widely used to sequence inserts in standard cloning vectors, we have assembled a list of primer sequences that you can order with a single click. For sequencing Gateway vectors, please use the Gateway-specific primers we have. M13/pUC. Analysis of sequences Alignment of the nucleotide sequences was conducted using the CLUSTAL W application [ , ] integrated in the BioEdit version 7.0.5.2 [ ] and then edited by hand. These free universal primers are being updated to reflect the needs of our customers. Figure 1. DNA Sequencing Standard Primers. GenScript offers FREE Standard Primers for DNA sequencing. Functionally tested for use in fluorescence-detection automated sequencing. 5'AGCGGATAACAATTTCACACAGGA3' 24. M13 is a filamentous bacteriophage composed of circular single-stranded DNA which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. The annealing region for the M13/pUC taa tac gac tca cta tag gg. Packaging Packaged in amber tubes to protect from light. 70. reverse primer(-48) 2. The main difference between PCR primers and sequencing primers is that the PCR primers are important for PCR amplification to obtain an amplicon, whereas the sequencing primers are important for sequencing a DNA fragment to reveal its nucleotide sequence. If you wish to sequence the both DNA strands you have to set 2 reactions with only one primer each. NEB offers its own series of these primers, shown in the NEB catalog. Our primer contains a "G" at the extreme 3' end, but the Gateway plasmids possess a "C" at this position. Use table search function or table pop-out (button above search bar) to better view our primer list. Primer Map Restriction endonuclease cut sites, and the protein translations of the DNA sequence can also be shown. Primer Sequence M13 Forward (-20) 5'{GTA AAA CGA CGG CCA G}3' M13 Reverse (-20) 5'{CAG GAA ACA GCT ATG AC}3' SP6 5'{ATT TAG GTG ACA CTA TAG}3' T3 5'{ATT AAC CCT CAC TAA AGG GA}3' T7 Promoter 5'{TAA TAC GAC TCA CTA TAG GG}3' T7 Terminator 5'{GCT AGT TAT TGC TCA GCG G}3' pcDNA3.1/BGH Reverse 5'{TAG AAG GCA CAG TCG AGG}3' 5'-pGEX 5'{GGG CTG GCA … Number of primers for a particular template the NEB catalog ( Ebersberg, ). Dna template are necessary to prime DNA synthesis for sequencing Gateway vectors, use... Escherichia coli primer … No … No primers for sequencing Gateway vectors, please use the malE primer ( #... Genewiz universal primer … No furthermore, two PCR primers ; the Forward and reverse primer are used a... ', with free hydroxyl groups at the tip of the host coli. Size ( mer ) Tm °C Remarks ; 1: you need to get rid of excess before. Primer M4 are Forward DNA sequencing primers while M13 primer M3 and M13 primer M3 M13. 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